FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238382
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGCGAATCTCGTATGCCGT23130.18677597058096776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5400.038.663358
GTCTCGC6000.034.7998281
TCGCTCT6200.032.513344
TATACTG2601.200533E-1030.4589255
GTCTTGC10800.029.9998471
CTGTCGC7250.029.7905469
CTCGCTC6950.029.0070483
TCTCGCT7350.027.4284322
CGCTCTG6850.026.2751165
GTTAACC2757.2868716E-926.1816863
GTATACT1959.240221E-625.8460251
GTATTAC2259.677624E-725.599871
TTATACT2259.684445E-725.5978034
GGTATAC1701.0811868E-425.4116361
TTGCTCT14650.024.571274
ACTTTCG1500.001556702323.997948
GTACTAA2151.968919E-523.4417421
GGGTATA3402.7139322E-923.2939991
GTCTAGA4950.023.272611
GTACTAG1902.2988877E-422.7367291
Produced by FastQC (version 0.11.5)