FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238382
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC5200.036.016131
ATACGGC800.002107969335.9972236
CTACGCT850.00283793833.8797384
TAGCACG900.003755045631.9975324
GGTATAC2301.0750227E-931.3183731
GTATTAG2901.2732926E-1129.8064521
TCGCTCT5900.029.285884
TCTCGCT6150.029.2660352
TACGCTC1000.006286265328.7977775
GCGTAGT1050.00795650127.4408631
TCTGTCG4550.026.9011968
GATCAAT2156.482842E-726.80271
GTGTTAG7950.025.3698521
GTCTCAC13200.025.102151
CCGCGCA1751.316345E-424.6858049
CTCGCTC6700.023.6399693
GTGTTAT2752.0964035E-723.5741941
CGCTCTG6150.023.4128285
GTATTAA3702.8194336E-1023.3618131
GTCCTAG4950.023.2831541
Produced by FastQC (version 0.11.5)