FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817255
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12100.040.459668
GTCTCGC12650.039.8437421
TCGCTCT14100.037.273694
TCTCGCT15100.033.8558732
CTGTCGC15450.032.1528329
GTCTTGC27600.031.827561
CGCTCTG15250.031.1582285
TACACCG2559.640644E-1131.0564045
GTATTAG9050.031.029061
CTCGCTC16300.030.479943
GTGTAAC8450.026.4154241
GTATTAT8000.025.201171
GTATTGG9250.024.9092621
TTAGACG2051.3633084E-524.583454
GTACTAC4800.024.0011141
GTTTCAC39950.023.9710733
GTCTTAC11950.023.4989971
GTCTCAC30200.023.12691
GGGTTTC44850.023.1181281
GTATTGT9450.022.8582041
Produced by FastQC (version 0.11.5)