FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817255
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12000.046.811021
TCTGTCG11000.041.8896188
TCGCTCT14600.037.47634
GTCTTGC24650.037.104071
CTGTCGC14250.033.851599
TCTCGCT15750.033.8257262
CGCTCTG15400.031.3220445
CTCGCTC16700.031.0393073
GTATTCG1753.9796414E-628.8067841
TTGCTCT34850.027.0622884
GTATTAA9100.026.9074331
GTCTTAC10350.025.7451921
GTATTAG8700.025.6612151
CTTGCTC33150.025.1924513
GTGTTAG18250.024.4660361
GTCTCAC32150.024.1923221
TTATACT7800.023.0749864
GTATACG2202.3489703E-522.9144861
CCGTTCG1600.002265825622.4993069
TTACTCT16150.021.8433654
Produced by FastQC (version 0.11.5)