FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907505
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTAGGCATATCTCGTATGCCGT32430.17001266051727257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7600.043.572218
GTCTCGC9250.036.581011
TCGCTCT10100.035.6380584
CTCGCTC10250.031.6073553
TCTCGCT11050.030.6221082
CTGTCGC9900.030.5407339
GTATAAC1902.2096901E-730.3134881
GTATTAT3950.029.1623421
GTCTTGC16450.028.447691
CGCTCTG10900.028.399285
TTGCTCT22750.025.3147684
GTTCCGC2401.6997492E-623.9981781
TCTGTCA23100.023.9962928
GTATAAA5150.023.7651881
GTCTAGC8550.023.5771561
CTGTCGT2151.9719218E-523.4382389
GTATAGA4002.910383E-1123.3982241
ACGTAAC1550.001884230823.2240433
GTACTAC2502.4240017E-623.038251
GTATATA4154.9112714E-1122.5525051
Produced by FastQC (version 0.11.5)