FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569339
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGACCAATATCTCGTATGCCGT39500.2516983264928737No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6400.032.621898
TTACGAC1050.00797330127.4294533
GACCCGT1959.248761E-625.8436876
GTCTTGC15100.025.2723351
TCGCTCT6700.024.7140624
ACCGATG1801.5949452E-423.9977118
CTCGCTC6950.022.7920993
CTGTCGC9350.022.329429
GTCCGGG3956.4392225E-1021.8741191
GTGTATA5350.021.5334011
GTCTCGC7100.021.296461
CGTCAGA3055.710208E-721.2465841
CCGTAGG1700.003222031521.174459
TCGCGCA1700.003222031521.174459
GTCTAAT3758.412826E-921.1206781
GTTTAGC5201.8189894E-1220.7698961
GGGTAGC5201.8189894E-1220.7698961
CGCTCTG7650.020.7039075
GGAGTGC37100.020.1813645
TTGCTCT20350.020.1651544
Produced by FastQC (version 0.11.5)