FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569339
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC5250.028.805611
GTCTTGC14350.026.5975131
TCGCTCT7050.024.5091634
TCTGTCG5850.023.3839538
GTGTTAC5900.023.1909561
TCGAACT10250.022.4774119
GTCTCGC7800.022.1581611
ACCCGCG1952.7415532E-422.153227
GGTATAC2303.31098E-521.9173131
TAGGGCG1650.002709100721.8168684
GTATTAA4307.8216544E-1121.771681
GTCTCAC16050.021.0882191
GTATCAA4151.1896191E-920.8233321
GTGTTAT5353.6379788E-1220.1908471
TGTACTT5353.6379788E-1220.1857015
GCCGCGT2557.342656E-519.7641478
TCACTCT18300.019.6709484
GTCCTAC4052.0352672E-819.5593621
GTATAGC2257.188118E-419.2037371
CCGCGTC2257.1989145E-419.1994559
Produced by FastQC (version 0.11.5)