FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2578840
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10950.034.845288
GTCTTGC25000.030.5262431
GTCTCGC11400.029.68251
GTATAAG7750.026.9403821
CTGTCGC15800.026.8829639
TCGCTCT13700.026.7998164
GTATTAA7450.026.0924571
ACCGTGT8600.025.9504858
CTCGCTC15150.022.8105683
GTCTTAC9500.022.7355331
GTCTCAC30250.022.6102681
TATACTG8050.022.3576585
GTGTAAT9700.022.2667561
GTGTAAC8150.022.0846161
TTCACCG8200.021.9486775
CCGTGTT9650.021.63489
GTATTAT6700.021.4912991
TCTCGCT14800.021.4041712
TAACACG5750.021.284494
GTGTAAA16100.021.0174241
Produced by FastQC (version 0.11.5)