FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326310
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8800.037.63048
GTCTCGC10150.034.04681
TCGCTCT11700.031.995624
GTCTTGC21150.029.2744771
CGCTCTG13600.029.1136635
CTATACG1000.006289879328.7954399
CTGTCGC11300.028.0309589
GTATTAA7450.028.0248151
GCGTAGT2105.305774E-727.4265861
TCTCGCT12800.026.9980452
CTCGCTC13200.026.7253363
GGTATAC4850.026.7197151
GTATTAG8100.025.775911
TAACACG4850.025.233664
TACACCG2351.416116E-624.5072845
TAATACT7400.024.3209954
GTACTAT6050.023.7999291
GTATTAT7450.023.1929511
GTGTAAG13100.023.0822981
TTGCTCT30000.022.7968794
Produced by FastQC (version 0.11.5)