FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326310
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10750.034.826518
GTCTCGC10500.033.6080441
CTGTCGC14050.028.1839229
CTCGCTC12350.027.9808773
TCGCTCT12150.027.8489384
CGCTCTG12400.026.7068825
TCTCGCT13250.026.080292
GTCTTGC20050.025.8615511
GTATTAG7850.025.6876771
GTGTTAG14300.023.1663821
GGGTAAG12150.021.9311751
CTACACG3305.0591552E-821.8158994
GTATAGA8600.021.7726541
TACTGCG2353.9166036E-521.4459467
TACACCG2353.9185517E-521.4445655
GTTTCAC35950.021.427523
GTATAGC5051.8189894E-1221.3912581
CTTGCTC27300.021.3604013
GTTCTAC7800.021.2358511
TAATACT7500.021.1177924
Produced by FastQC (version 0.11.5)