FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537426
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10850.051.7596138
CTGTCGC15200.036.473159
GTCTTGC25350.035.235081
GTCTCGC10350.034.7985571
GTCTTAC9500.032.6044161
TCGCTCT11650.030.2828184
GGTATAC5050.028.5279251
TTGCTCT32100.028.4855634
TCTCGCT12450.026.6146072
GTATAAC4750.025.7802351
TATACTG7300.025.643465
CTTGCTC34350.024.3255583
GTATTAG7950.023.5579681
GCTCTGT60300.023.4026436
GGGTTTC40950.023.3953361
CTATACT5550.023.3510114
GTGTAAA14900.023.2052631
TTACTCT14650.023.0986374
TCTTGCT37650.022.958732
GGAGTGC66000.022.7996675
Produced by FastQC (version 0.11.5)