FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154214
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10950.048.6783331
TCGCTCT11800.041.4909554
TCTCGCT12950.040.6042252
TCTGTCG10050.039.4024478
CTCGCTC12800.038.829093
GTCTTGC20400.034.9561121
CTGTCGC12500.033.4075439
CGCTCTG14450.030.892315
TATACTG5750.030.0517625
TTCACCG7400.029.188795
ACCGTGT8850.028.4741868
GTTTCAC33700.027.3588713
TTGCTCT27500.026.9669024
GTGTAAC6700.026.8771571
TACCCGT1358.419936E-426.6663025
GGGTTTC38200.026.0215911
GTATTAG6400.025.886061
GTATATA10300.025.1758081
CCGTGTT8950.024.9382069
GTATTGA6750.024.5438211
Produced by FastQC (version 0.11.5)