FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306233
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9350.049.282578
CTGTCGC10850.039.1513989
TCGCTCT10350.038.260214
GTCTCGC9550.037.7161251
GTCTTGC21850.033.2989351
TCTCGCT11500.029.4415382
CGCTCTG12550.028.1110695
GTCTTAC9250.028.0363331
CTCGCTC12600.028.0146983
GTATAAT6750.027.7478941
GTCTCAC28600.027.7068461
GGGTTTC39400.027.6083951
GTGTAAC6900.027.1446781
TTCACCG7700.027.1164155
GGGTACG3204.5474735E-1127.0141751
GTTTCAC37500.026.1257083
ACCGTGT8300.026.0236438
TCACCGT9150.025.1798926
TTGCTCT31300.025.0730484
GTATTGC6600.024.01261
Produced by FastQC (version 0.11.5)