FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306233
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.044.9122438
GTCTCGC10200.043.7258031
TCGCTCT11450.040.2052844
CTCGCTC12450.033.509453
CTGTCGC14600.033.0088659
GTCTTGC22550.032.857691
TCTCGCT13050.032.519972
AATACCG1601.9979198E-631.4692735
CGCTCTG13350.030.1727875
GTATCGC1204.2497148E-429.9733351
GTACCGC1453.683543E-529.7666241
GTTCGGA1504.641895E-528.7744031
TCGTACT1306.791953E-427.6652974
GGTATAC4500.027.1758251
GTATAAT7300.026.6064661
GTTTCAC34850.026.4189663
TTGCTCT29300.026.2678684
GTATTAC6050.024.9695221
GTATTAT8600.024.257491
GTACTAA5050.024.2160821
Produced by FastQC (version 0.11.5)