FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281636
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9950.044.8864751
TCGCTCT11150.037.4525224
TCTGTCG9100.035.604028
TCTCGCT12300.035.1392942
CGCTCTG13750.030.8942225
CTCGCTC13600.030.191373
CTGTCGC11550.029.2983939
GTATTAG7900.029.1789571
GTCTTGC21500.029.1492711
GTATTAC4500.028.814221
GTATTAA6650.028.1642741
GTACTAG5100.026.8367751
TAATACT5300.025.8110494
GGGTTTC40450.025.4662171
GTTTCAC38050.025.1793123
GTACGGT2051.3571369E-524.5975063
GTATTAT6450.023.4534361
GTATATA8700.023.1838571
GTGTTAG17650.022.4473381
GGTTTCA40500.022.2331922
Produced by FastQC (version 0.11.5)