FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281636
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10500.039.0474788
GTCTTGC21400.033.2772871
GTCTCGC9850.032.8626631
TACACCG1902.2275526E-730.2861355
GTATTAC4550.030.037841
TCGCTCT11700.029.5095654
GTATAAC4700.029.0791851
CTGTCGC15300.028.6778039
TTGCTCT28550.025.9500714
GTCTCAC28150.025.5533661
CGCTCTG13350.025.3235195
GTTTCAC36500.024.830483
CTCGCTC13750.024.5868343
ATACTAT6750.024.5093356
GTGTTAG15000.024.4571231
GGGTTTC40950.024.4167231
GTGTAAC6800.023.272351
GGTATAC4353.6379788E-1223.150761
GTATTAT6350.022.6559751
TCTCGCT14400.022.477992
Produced by FastQC (version 0.11.5)