FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220739
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8550.043.7889868
GTCTCGC11500.035.701831
TCGCTCT10900.035.6693274
GTCTTGC22200.035.041631
CTCGCTC11700.032.6289833
CTGTCGC12350.030.8984419
TCTCGCT12200.030.1109092
GTCTCAC26100.029.253571
TCTGTCA27050.028.4802768
TTGCTCT27950.026.27524
GTGTTAG15800.025.5296251
CGCTCTG14750.024.894645
TCACTCT28500.024.5049924
CTTGCTC28550.024.4725443
GTATAGT6050.023.8115751
GGTATAC3702.8194336E-1023.3610861
TCTTGCT32400.022.8984322
CTGTCAC33350.022.8843029
GTTTCAC33150.022.597653
CCGTAAA1600.002259790222.509381
Produced by FastQC (version 0.11.5)