FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220739
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10850.037.7881288
GTCTTGC21150.037.755611
TCGCTCT11700.035.6576234
GTCTCGC10900.034.979881
GGTATAC3400.033.8541
TTGCTCT25050.031.8731884
GTATTAC4800.031.473641
TCCGTCG1153.3131795E-431.2739288
GTGTTAC8700.030.5950661
CGCTCTG13750.028.2488865
CTGTCGC14950.027.4248289
TCGGATA1050.00801316927.4019172
TCTCGCT14000.027.2306562
CTCGCTC14600.027.0969283
CTTGCTC28550.026.706073
GTGTTAG13950.026.3005541
GTCTCAC28700.026.068761
GTGTAAC6450.025.6529351
GTGTTAA8200.025.4421061
TCTGTCA27750.025.4023198
Produced by FastQC (version 0.11.5)