FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271270
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10650.040.5853161
TCTGTCG10050.039.402598
TCGCTCT12000.036.001234
GTCTTGC22550.031.6268481
CTGTCGC14100.030.127369
TCTCGCT14150.029.0045222
CGCTCTG12800.028.6878455
CTCGCTC13800.027.6531163
GTGTAAC6550.027.4957751
GTGTACG2405.6146746E-827.0145991
ATACCGT1358.419894E-426.66646
GTCTTAC9700.025.9934271
GTATTAG8150.025.6334861
GTGTTAC9700.024.508091
GTGTTAG15600.024.4747641
CTTGCTC27500.024.0880953
TAGTACT5700.024.0008184
GTGTAAA13400.023.6545751
TTGCTCT30550.023.5687264
GTATAAG7400.023.363981
Produced by FastQC (version 0.11.5)