FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1894705
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9300.046.4527558
GTCTTGC16950.038.67931
GTCTCGC8300.036.456761
CTGTCGC11950.036.1515169
TCGCTCT9900.033.4544834
CCGTTAT1255.3488323E-428.8182011
GTATTGG5300.027.1869831
GGGTTTC32100.027.1573411
TTGCTCT24450.027.0919744
TACATCG1358.418976E-426.6666185
GTTTCAC29000.026.5654683
GTATTAG6050.026.1983641
TCTCGCT10750.026.1195052
GTATAGG5250.026.073611
GGTATAC4150.026.0405451
GTATAGA6400.025.8913521
GTACTAT4200.025.7305371
GGGTAGT5800.024.8432771
CTTGCTC24100.024.497883
CTCGCTC11200.024.4285263
Produced by FastQC (version 0.11.5)