FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133830
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7850.039.4356548
GTCTCGC10750.036.500911
TCGCTCT10550.036.178124
GTCTTGC20300.034.721151
CTGTCGC10850.031.8494369
TCTCGCT11750.030.6525552
CTCGCTC12350.029.1633633
GTATAAG5800.028.8149781
GTGTTAG15600.026.5502551
GTATCGT1100.00956811426.4232181
CGCTCTG12550.026.3878695
GGATACG1751.2372622E-424.913321
GTACGTA1751.2372622E-424.913321
GTCTCAC24350.024.7683751
GGGTAAC8000.024.5240481
GCGTTGC1801.4979512E-424.2212831
GTAATAC4800.024.0111683
TATACTG6600.023.9976655
GTATTAC5200.023.755491
GTGTAAC6200.023.4399531
Produced by FastQC (version 0.11.5)