FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999155
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9950.057.8841258
CTGTCGC11500.052.586479
GTCTCGC9300.044.5314981
GTCTTGC18700.039.6309051
TCGCTCT9750.037.668444
TCTCGCT10400.037.398952
CTCGCTC11550.033.0516323
GGTATAC3350.030.3639761
TAGTACT4900.027.9234854
CGCTCTG12450.027.1782255
GTACCGA1100.00957258626.4206051
GTCTCAC25250.025.8974251
CTTGCTC26850.024.6798523
GTATTAT5300.024.6758461
GGGTTTC32800.024.5880761
GTGTAAC5400.024.2188851
TTGCTCT28300.024.1740424
GTCTTAC7850.024.0646271
TAATACT4850.023.7569314
GTGTTAC8050.023.4667451
Produced by FastQC (version 0.11.5)