FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999155
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9250.051.395871
TCGCTCT10200.044.4703754
GTCTTGC17500.041.1611981
TCTCGCT11700.036.9229052
TCTGTCG8650.036.6241048
CGCTCTG11950.035.547955
CTCGCTC12350.033.8136063
ACCGTGT5700.031.5787988
CTGTCGC11800.029.8981679
GTATTAC3900.029.5516281
GTGTTAG12300.026.9388311
GTATTAA5150.026.5749471
GTTTCAC28950.026.1138693
GTATAAC3900.025.8576741
GGGTTTC32350.025.8291281
GTCTCAC22600.025.816811
TTGCTCT26500.024.9961074
GTGTTAT8550.024.4319381
GTATAGA6250.024.2027851
GTGTAAT7550.023.8516881
Produced by FastQC (version 0.11.5)