FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375830
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6050.051.1730778
GTCTCGC5600.043.736131
GTCTTGC14000.043.221591
TCGCTCT6100.041.3140954
CTGTCGC9150.038.5570149
GTACCGT800.00210209136.0179941
TCTCGCT6450.034.6068422
GTACTAA3200.031.5157431
GTATTAG4950.030.5607191
GTTCGGA950.00487873130.3309421
CCGTTCG950.00489089130.3155089
CTCGCTC8200.029.855553
TTGCTCT20000.028.0817784
CGCTCTG8550.027.7892155
GGGTTTC23600.027.1661131
AACCGTC1358.418169E-426.6664167
CTTGCTC19700.026.3163873
GTGTAAC5300.025.824221
TACACCG1400.001040896825.7140455
GTGTACG2001.1202623E-525.2125931
Produced by FastQC (version 0.11.5)