FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878009
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7950.040.7508288
GTCTCGC8700.035.07751
TCGCTCT9600.034.504984
GTCTTGC18550.034.469691
GTATAGC4200.032.8702431
GTCTTAC9700.032.2103421
TCTCGCT10050.030.8219052
GCGTTAT950.0046774530.5939121
CGCTCTG10150.030.4995525
CTGTCGC9850.029.9666869
TACACGA1753.969999E-628.8148982
CTCGCTC10050.028.671543
GTACTAA4550.025.5509591
GTGTTAG13050.024.4985731
GTGTAAC6600.024.220181
TTGCTCT26300.024.094734
GTCTCAC23200.023.4893991
GTATTAG6250.023.2513731
GTGCTAC7550.023.097391
TTACTCT12800.023.0658264
Produced by FastQC (version 0.11.5)