FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033326
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.049.87288
GTCTCGC10000.045.3864441
TCGCTCT11700.041.845844
GTCTTGC17400.041.8174671
CTGTCGC13500.038.399719
CGCTCTG12750.036.1409075
CTCGCTC13100.034.076083
TCTCGCT12750.033.88212
TAATACT6100.030.6882934
GTACTAG3850.028.0683021
ATGGCGT8850.026.8472566
GTATTAG7400.026.2855871
TTGCTCT27050.026.0848334
GTCTCAC24950.025.698341
GTGTTAC7600.025.593861
GTGTAAC5650.025.5015851
GTGTTAG13850.024.9676151
GTATTAC5200.024.9376071
CTTGCTC25250.024.5225873
ATACGAC1500.001556335623.999823
Produced by FastQC (version 0.11.5)