FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944483
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9000.051.1958058
CTGTCGC11200.039.211079
GTCTTGC18200.033.534161
TCGCTCT9700.031.1815244
GTCTCGC10300.030.3326841
GTTTCAC30350.029.901893
GTGTAAC7050.028.8568211
GTCTTAC7900.028.5111141
GGGTTTC33050.028.3594511
GTATTAG6750.026.9101331
CTCGCTC10850.026.553213
CGCTCTG11700.026.459375
TTCACCG6650.025.9828325
TCTCGCT11100.025.9551642
TAGTACT4200.025.7194544
CCGTGTT7600.025.5768499
TACCCCG2001.1256385E-525.1979335
TTACACT9200.024.2657454
ACCGTGT8950.024.1321018
GGTTTCA34350.024.1133692
Produced by FastQC (version 0.11.5)