FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944483
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10000.043.22831
TCTGTCG8650.040.7855538
TCGCTCT9200.040.697174
TCTCGCT11400.034.105662
GTCTTGC17700.033.3777771
CGCTCTG10900.031.0454335
CTCGCTC12100.030.348243
CTGTCGC12200.030.0987119
ATAGTAC5200.027.693343
TCTCGAT9400.027.574087
GTGTACG2654.8785296E-927.1876091
GTGTTAG13300.027.0853981
GTGTTAC7600.026.543691
CTCGATC9100.026.1095228
TCGATCT8650.025.8037689
GTATTGG6200.024.4030691
GTCTTAC8050.024.1648851
GTATTAA7200.024.0157181
GTGTAAT7800.024.0157181
TTGCTCT23750.023.9503694
Produced by FastQC (version 0.11.5)