FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1793752
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7700.046.7481048
GTCTCGC8950.040.590151
CTGTCGC9450.039.614699
CGTAGAT750.001531858338.4161263
TAGTACG1157.4832496E-637.572614
TCGCTCT8850.035.8036544
GTACGGA1655.9082595E-835.227331
GTACTAG3300.033.025621
TCTCGCT11000.030.7765542
TCGCACT1453.6558984E-529.7989654
CGCTCTG10600.029.2043365
CTCGCTC11100.029.2014473
TATACCG1000.00628801528.7968315
GTCTTGC18100.028.099151
TATACTG4900.027.9152985
GTCTCAC17900.026.3835981
GCGTAAA1409.872753E-425.9487041
GTGTTAG12150.025.7137781
GTAGCAC5750.025.0539953
GTGTTAT6800.024.574951
Produced by FastQC (version 0.11.5)