FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1897688
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8200.049.1668788
CTGTCGC10250.042.143049
GTCTCGC7600.041.0963971
GTCTTGC17650.034.1572041
TCGCTCT9400.032.170244
GTGTAAA11950.030.391421
GTATTAG6550.028.8324091
GTGTATA9150.028.5778981
CGCTCTG9850.028.5053835
GGTATAC3600.028.2471351
TCTCGCT10700.027.5982592
GTCTTAC8000.027.238311
TAATACT5350.026.9159094
GTGTTAG12800.026.6708451
GTATTAT5200.026.5398921
GTATTAC4950.026.4129071
GTGTTAC7750.026.24251
GTTATAC3358.185452E-1125.7999023
GTGTTAT6800.025.6360571
CTCGCTC10850.025.2252483
Produced by FastQC (version 0.11.5)