FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1897688
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7450.046.3884968
GTCTCGC8500.044.0683941
TCGCTCT10300.036.3498734
GTCTTGC18250.033.155781
CGCTCTG10350.032.6959765
TCTCGCT11450.032.0701872
CTGTCGC11600.031.0339729
GTATTAG6150.029.282471
GTGTAAC6450.029.0373151
GTCTTAC7550.028.6231331
CTCGCTC12650.028.4587783
CTCGATC6950.027.9707648
GTGTTAG11400.025.2753941
TCGATCT8050.025.0430669
GTGTAAT8300.024.3009221
GTATTAA5400.024.0116271
GTCTCAC25100.023.82031
TTACTCT11200.023.785954
GTCGTAC1550.001878097423.2370571
GTCCTAC6600.022.9201871
Produced by FastQC (version 0.11.5)