FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126689
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10150.049.6538358
GTATCGC901.3791323E-648.0495451
GTCTCGC10000.042.5238461
GTCTTGC22550.038.9936451
CTGTCGC13800.037.042489
TCGCTCT10900.036.995054
CGCTCTG11450.035.2130245
TCTCGCT12150.032.613952
GTACTAG3900.031.4170111
TTGCTCT28200.030.8971424
GTACTAC3500.030.888991
GTACACG2951.6370905E-1129.3183651
CTCGCTC13300.029.252243
TACCCGT1306.754207E-427.691566
GTATTAG6800.027.5578251
GTATTAA6800.027.5578251
GTCTTAC8450.027.2944151
GTGTTAG14550.026.749231
GTCTCAC25450.026.3375681
GTATAAT5750.026.3227941
Produced by FastQC (version 0.11.5)