FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919654
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9150.050.3593188
GTCTCGC8650.049.9929541
TCGCTCT9800.047.028964
CTCGCTC11050.041.0700763
CTGTCGC11950.040.3671269
TCTCGCT11350.037.4458242
CGCTCTG11200.035.35625
GTCTTGC18750.033.8263441
GTATAGC4500.032.032521
TAATACT4950.029.0961974
TAGTACT4000.027.004914
GTATAAC3006.2937033E-1026.4268321
TTGCTCT25050.025.8729674
ATACTGA7650.025.4110896
GTATTGA5700.025.2888361
CTTGCTC25150.024.918883
GTTCGGT1450.001273677224.8398673
TCGATCT7250.024.8269259
GTGTTAG13150.024.6638241
CTCGATC7300.024.656888
Produced by FastQC (version 0.11.5)