FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369919
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9100.045.925661
TCTGTCG9000.044.798768
TCGCTCT11100.039.5698134
TCTCGCT12250.037.0408332
GTCTTGC22650.034.6758461
CTCGCTC13300.032.491963
CTGTCGC12800.030.9366449
CGCTCTG12750.030.4932755
GTGTTAG16200.027.1321131
GTGTTAC10000.026.6606371
GTCTCAC28450.025.3271621
TTGCTCT30050.024.9198824
GTTTCAC35050.024.2476843
GTATTAG6900.024.0185931
GTGTAAC7350.023.5284181
GGGTTTC38750.023.4297491
TCTGTCA30750.023.4139848
GGTATAC5250.023.3323461
GGCGTAC1550.001875068223.24381
GTGTATA9700.023.0281351
Produced by FastQC (version 0.11.5)