FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802903
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11350.041.2325068
GTCTCGC12250.041.171261
TCGCTCT13650.036.926164
GTCTTGC27550.033.7365231
CTGTCGC15200.032.6834379
CTCGCTC16350.029.9560013
TCTCGCT16600.029.9387512
GTATTAA10000.029.5403791
CGCTCTG17100.029.0519475
GCGTAGT3301.8189894E-1228.3832191
TTACACT15000.024.962084
GTATTAT8600.024.2958341
GTGTTAT10300.023.7833981
GTTTCAC42600.023.1634733
GTATAAT10900.023.1352271
GGGTTTC47500.022.9042241
TACACTG19350.022.697145
TTGCTCT38700.022.3274444
TCTGTCA37200.022.2575388
TTCACCG7800.022.153325
Produced by FastQC (version 0.11.5)