FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3379712
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15300.036.2349938
GTCTCGC15350.034.244811
TCGCTCT16250.032.7879034
GTCTTGC31900.030.4735741
CGCTCTG17200.028.883915
CTGTCGC21100.028.3224589
CTCGCTC18600.027.4840493
TCTCGCT20350.026.1827622
GTGTTAG23900.025.91081
GTACTAA7900.023.698811
GTACAGG21400.022.5445371
GGTATAC7350.022.533091
GTCTCAC40350.022.4857461
GTATAAC7700.022.4440291
GTTAGCC19200.022.1251413
GTGTTAC17000.021.6023771
GGGTTTC58150.021.5466521
CTCGATC13400.021.4923578
TTACACT17200.021.3489764
TTGCTCT47050.021.2711244
Produced by FastQC (version 0.11.5)