FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583569
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11000.041.2361648
GTCTCGC11100.036.329071
GTATTAC5800.036.0047041
TCGCTCT12550.034.9958464
GTCTTGC23700.034.3336031
CTCGCTC13550.029.7563133
GGTATAC4600.029.7430151
CTGTCGC16150.028.978199
GTACTAG4300.028.4688361
TAATACT7050.027.5743354
TCTCGCT14300.027.1902582
TACTCCG2955.2386895E-1026.847335
CGCTCTG15050.026.3121645
GGGTTTC44850.026.1706451
GTGTTAG18350.024.7225761
GTTTCAC40750.024.5593913
GTACTAC5050.024.2407911
GTGTTAC11400.024.0031381
GTGTAAA15600.024.0031381
TTACACT12300.023.9998844
Produced by FastQC (version 0.11.5)