FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3277911
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14250.046.9891058
GTCTCGC14500.045.215511
TCGCTCT17150.039.044024
CTGTCGC18550.038.424999
TCTCGCT16800.037.7381672
CGCTCTG17000.035.5767365
GTCTTGC31350.034.7019121
CTCGCTC18450.033.582233
GTATTAG11250.030.7399231
GTATAAC7600.027.49151
GTTTCAC52650.027.0940693
CTTGCTC39600.026.1983973
GTGTAAC11000.025.5438271
GGGTTTC60900.025.4352021
GTCTTAC14700.024.5056781
GTGTAAA21650.024.292881
GTATAAT9600.024.0155641
ACCGTGT11950.023.4977258
TTGCTCT42700.023.4381144
GTATAGG10250.023.195521
Produced by FastQC (version 0.11.5)