FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3277911
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14700.046.0405928
GTCTCGC14150.038.166431
TCGCTCT16800.034.285554
GTCTTGC32650.033.3020131
CTGTCGC20250.032.7109539
CTCGCTC17800.031.1459163
CGCTCTG18650.030.4985085
GTATTAC8350.030.1827161
TCTCGCT18750.028.8011782
GTATACG2654.9112714E-927.1725791
TAGTCCG1358.420443E-426.6665385
GTGTTAC13250.025.5422251
GTATTAG12800.025.315081
GTCTCAC39050.024.7093031
GTTTCAC50650.024.592183
TTGCTCT43350.024.4151044
GTCTTAC15100.024.320361
GTACTAC6900.024.0024451
CTTGCTC42350.023.8015383
GTACTAT8700.023.1747781
Produced by FastQC (version 0.11.5)