FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3233868
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13000.037.107368
GTCTCGC14050.035.383191
TCGCTCT16000.030.1506654
CTGTCGC17900.028.9601889
GTCTTGC30700.028.3969171
TCTCGCT17350.027.4078732
CTCGCTC17750.026.7902343
CGCTCTG18050.026.3274485
GTCGTAA1100.00997388826.199411
CGTTTCG1400.001041292725.713669
GTATAGA10650.025.7074011
GTATAAC7050.025.5490681
GTATTAA10700.024.2405741
TTGCTCT40450.023.496194
GGTATAC6750.023.4824331
GTATTAG12100.023.2222021
GTGTTAC16500.023.142811
GTTACCC12200.023.0322133
GTATAAT9950.022.4472311
GTTTCAC52000.021.7534053
Produced by FastQC (version 0.11.5)