FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3233868
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15400.040.6743248
GTCTCGC13400.037.079451
CTGTCGC19750.033.175929
GTCTTGC31300.031.5184761
TCGCTCT15450.028.8938354
GTGTTAC13300.026.5297661
TCTCGCT17750.026.3675882
GGTATAC6450.025.6777571
GTATACG2259.674313E-725.6033291
CGCTCTG16900.025.5626875
TTACACT17850.025.008954
GTACTAC6100.024.790111
CTCGCTC17850.024.6055773
GTATTAA10800.024.0031221
GTGTTAG25750.023.4904331
TACACTA8400.023.1433625
GTCTTAC14200.022.8198681
GTGTAAT13300.022.73981
GTATTAG12550.021.8036331
TTGCTCT47150.021.684464
Produced by FastQC (version 0.11.5)