FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3582837
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14300.037.761678
GTCTCGC14800.036.491031
GTCTTGC33650.035.095011
GTATAAC7450.033.8297161
TCGCTCT15950.030.6958924
CTGTCGC19350.029.0232269
GCGTTAC2405.639413E-827.0033651
CTCGCTC17150.026.0291253
GTATTAA12250.025.8644451
GTGTAAC10750.024.7844811
GGGTTTC57550.024.3992161
TCTCGCT18700.024.2580132
CGCTCTG19000.024.2526055
GGTATAC6850.024.1781941
TTGCTCT45950.023.9738584
GTATTAG12850.023.5360051
GTCTCAC40450.023.320581
TAATACT10650.022.985894
GTGTTAC14800.022.8677141
GTTTCAC49900.022.5089933
Produced by FastQC (version 0.11.5)