FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3145137
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13800.043.3058248
TCGCTCT15050.042.580884
GTCTCGC15700.040.3831061
CTCGCTC16700.034.9466823
CTGTCGC18300.033.0482069
TCTCGCT19250.031.8145372
CGCTCTG18300.031.4774845
GTCTTGC31700.031.3643671
GTCTTAC13850.026.529991
GTGTTAC13950.025.3068751
GTTTCAC46900.025.041033
GTTAGCC16850.024.8007873
GTGTTAG21700.024.5690671
GGGTTTC51300.024.1561531
GTGTAAA21150.023.5047381
TTGCTCT42700.023.1022244
TCTCGAT13000.022.7084677
CTTGCTC42650.022.6372243
GTATTAT11850.022.4957281
GTGCGTA2903.4955337E-722.3594531
Produced by FastQC (version 0.11.5)