FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2986012
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12100.041.057118
GTCTCGC14800.035.0481031
GTCTTGC29450.034.4927331
TCGCTCT15150.033.7419624
CTGTCGC16900.029.3959169
TCTCGCT17350.028.6512372
CTCGCTC17550.028.3247283
CGCTCTG17100.028.2100165
CGTTTCG1607.163051E-526.9995129
GTACTAC5150.026.5790941
GTATAGA9150.025.982841
TTGCTCT40550.025.923084
GTATAGT9300.025.563761
GTATTAC6650.024.9172421
GTGTAAA18800.024.9085981
GTGTTAG21500.024.7963091
GTATTAT10000.024.494731
GGGTTTC52350.024.0832521
GTATAAG9650.023.8900171
GTTTCAC47850.023.6380423
Produced by FastQC (version 0.11.5)