FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2890764
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11700.038.7684948
GTCTCGC12200.037.2053641
CTGTCGC13700.031.5322489
TCGCTCT14450.031.3909474
GTCTTGC28750.031.0748231
TCTCGCT15700.029.370082
TAATACT9400.029.1063354
CGCTCTG16700.026.2993585
GTCTTAC10000.025.9374541
TTCACCG8400.025.7142415
CTCGCTC17100.025.7015063
TTACACT13700.023.6495954
GTATTAG9850.023.4066121
GTATTAT8050.023.2703191
TAGTACG1550.001883651523.2257674
CGTTTCG1550.00188384123.2253659
GTACTAG6250.023.0555131
GTATAAC5950.023.0070781
GTGTAGA15700.022.4864671
GTGTTAT11900.022.4016281
Produced by FastQC (version 0.11.5)