FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3493488
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15300.033.410868
GTCTCGC13100.030.7813151
TCGCTCT15950.029.7935754
GTCTTGC29500.029.5347841
GTATTAA8400.024.8593161
TTGCTCT40600.024.4732934
CGCTCTG16950.024.212775
CTGTCGC23300.023.4856859
TATACCG1851.9196946E-423.3517235
GTCTTAC12900.022.885721
CTCGCTC17100.022.3161413
GTACTAC4256.730261E-1122.0254551
GTCTCAC32900.021.8864121
TAATACT7900.021.8737644
GTCTAAC6300.021.7161831
TCTCGCT18400.021.5226972
CTTGCTC40350.021.0560953
GAGTGCA80050.020.5075076
GTGTTAC11300.020.3911631
GGCTGGA105250.020.387531
Produced by FastQC (version 0.11.5)