FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526394
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8850.047.1845938
CTGTCGC12400.036.5792129
TCGCTCT12600.036.5699964
GTCTCGC12950.035.052711
GTCTTGC25550.033.840841
GTATAAT6600.030.5678521
CTCGCTC14800.029.6921443
TCTCGCT15650.029.0001092
CGCTCTG14050.027.159085
ACCGTGT8850.026.8464078
GTATAGG7150.026.2010151
GTCTTAC9000.025.618771
GTATTAG8250.025.3276481
TTCACCG7700.025.2457645
GTTTCAC40100.024.6121543
GTATAAG8650.024.156431
GTGTAAA15000.024.0175971
GTATTAC4850.023.7699951
GTATAGC6400.023.6423231
TTACACT12650.023.3350544
Produced by FastQC (version 0.11.5)