FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526394
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11700.047.9245221
TCTGTCG10050.039.3425838
TCGCTCT13950.038.6503374
TCTCGCT15150.036.5379642
CGCTCTG14750.034.117115
CTCGCTC16650.031.5191763
GTGTACG3500.030.808621
GTCTTGC23000.030.630021
CTGTCGC13550.030.2401969
GTCGTAC1204.266902E-429.9528261
GTCTTAC10300.029.3130571
GTGTAAC7400.029.1432911
GTGTTAG16850.028.584061
GTACGAT1607.238858E-526.9575441
GTATTAG9250.024.8689421
GTTTCAC37950.024.8156573
TACACCG1450.001288937824.7895285
GTATAAG6800.024.3146481
TACACTT7700.024.2744185
GGTATAC5100.023.9622611
Produced by FastQC (version 0.11.5)