FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2725014
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11950.039.1057858
GTCTCGC11750.033.040261
GTCTTGC25450.032.4861071
CTGTCGC15350.031.8490229
TCGCTCT13750.029.2785114
TCTCGCT15700.027.473582
CTCGCTC15500.025.9728763
GTCTTAC11950.024.6662731
GTACTAC5050.024.2016581
GTATTAG8500.023.6824531
ATGGCGT11450.023.2314266
TAATACG1550.001900552123.1900654
CGCTCTG14450.022.8851455
TTGCTCT39000.022.4884194
GTGTTAG19650.022.3179781
AATGGCG13850.021.8003375
GGGTTTC46900.021.4606461
TACACTG17500.021.3613645
GTGTAAA17950.021.2275091
GGCGTAC1700.003248702721.1450481
Produced by FastQC (version 0.11.5)