FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597649
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11350.052.6509068
CTGTCGC14350.046.1593329
GTCTCGC11250.042.9096831
GTCTTGC27300.039.0599861
TCGCTCT12800.035.436784
TCTCGCT13700.034.175672
GTCTTAC11250.032.6625941
CGCTCTG12900.031.8133035
CTCGCTC14200.031.4505063
GTATTAC7100.030.4435961
TTGCTCT37000.030.1615474
GTATTAT7900.027.3606991
CTTGCTC36300.027.1855563
GTCTCAC33900.026.7795871
TCTTGCT40150.025.834572
GTATAAT8950.025.7608361
GTGTATA14750.025.4006211
GTATAGG8050.025.0608161
GTTTCAC40300.025.0234553
TACTCTG18600.024.3865995
Produced by FastQC (version 0.11.5)